Journal: bioRxiv
Article Title: Modulating Nucleic Acid Phase Transitions as a Mechanism of Action for Cell-Penetrating Antimicrobial Peptides
doi: 10.1101/2023.03.11.532143
Figure Lengend Snippet: AMP effects on transcription and translation processes. Cell-free eGFP synthesis followed in the absence and presence of antimicrobial peptides (a-f). The cell-free synthesis of eGFP was initiated by supplementing CFPS solution with 6 ng/μL pDNA (a-c) or 80 ng/μL mRNA (d-f) encoding eGFP protein. Continuous lines are the fits (see Materials and Methods). Comparison of EC50 values of AMPs in pDNA- or mRNA-initiated CFPS reaction (g). Error bars are the standard deviations.
Article Snippet: Every precaution was taken to avoid contamination with RNases, and RNA samples were kept as 10 μL aliquots at −80 °C to minimise degradation. pDNA harbouring gene encoding eGFP was purchased from GenScript.
Techniques: Comparison